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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, <t>and</t> <t>ERK1/2</t> proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.
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Image Search Results


Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-BSND (1:750) , Novus Biologicals , Cat#: NBP2-49101.

Techniques: Transduction, Recombinant, RNA Sequencing, Software, Cell Attachment Assay

Journal: Cell reports

Article Title: A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs

doi: 10.1016/j.celrep.2023.112623

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-BSND (1:750) , Novus Biologicals , Cat#: NBP2-49101.

Techniques: Transduction, Recombinant, RNA Sequencing, Software, Cell Attachment Assay

Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, and ERK1/2 proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

Article Title: Epithelial cell transforming 2 is regulated by Yes-associated protein 1 and mediates pancreatic cancer progression and metastasis

doi: 10.1152/ajpgi.00185.2020

Figure Lengend Snippet: Role of ECT2 in PI-3 kinase and STAT3 signaling. A: Western blot analysis of the p85 subunit of PI-3 kinase, Akt, STAT3, and ERK1/2 proteins in control and ECT2-deficient AsPC1 cells. The data were developed using the same set of AsPC1 cell lysate and shown with the same loading control as in Fig. 5A. B and C: qPCR analysis of IL-11 or IL-6 in control and ECT2-deficient AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). D and E: qPCR analysis of IL-11 or IL-6 in control and YAP1 knockdown AsPC1 or MIA PaCa-2 cells, respectively (*P < 0.05). ECT2, epithelial cell transforming 2; PI-3, phosphoinositide 3; YAP1, Yes-associated protein 1.

Article Snippet: Antibodies The antibodies and related reagents used in this study include: anti-ECT2 (sc-1005) ( 57 ), anti-GAPDH (sc-365062), and anti-YAP1 (H-125, sc-15407; H-9, sc-271134) antibodies and protein G PLUS agarose (SC-2002) from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phospho-S127 YAP1 (Cat. No. 4911; 13008 P), anti-phospho-Erk1/2 (Cat. No. 49101), anti-Erk1/2 (Cat. No. 9107), anti-phospho-p85 Y458 (Cat. No. 4228), anti-p85 subunit of PI-3K (Cat. No. 4292), anti-phospho-Akt S473 (Cat. No. 9271), and antiphospho-STAT3 Y705 (Cat. No. 9131) from Cell Signaling Technology (Danvers, MA); anti-YAP1 (NB110-58358) from Novus Biologicals (Littleton, CO); anti-Ki67 (ab15580) from Abcam (Cambridge, MA); anti-α-smooth muscle actin (α-SMA; A2547), anti-HA agarose (A2079), and anti-FLAG agarose (A1205) from Sigma (St. Louis, MO); and anti-Ki67 (7B11) from ThermoFisher Scientific (Waltham, MA).

Techniques: Western Blot